Publications

Publications

Invadopodia Are Required for Cancer Cell Extravasation and Are a Therapeutic Target for Metastasis

By:
Contributors: John D. Lewis Research Group, Katia Carmine-Simmen, PhD, Konstantin Stoletov, PhD

Cell Rep. 2014 Sep 11;8(5):1558-70. doi: 10.1016/j.celrep.2014.07.050. Epub 2014 Aug 28.

Leong HS1, Robertson AE1, Stoletov K2, Leith SJ1, Chin CA1, Chien AE1, Hague MN3, Ablack A1, Carmine-Simmen K2, McPherson VA1, Postenka CO3, Turley EA4, Courtneidge SA5, Chambers AF3, Lewis JD6.

 

Abstract
Tumor cell extravasation is a key step during cancer metastasis, yet the precise mechanisms that regulate this dynamic process are unclear. We utilized a high-resolution time-lapse intravital imaging approach to visualize the dynamics of cancer cell extravasation in vivo. During intravascular migration, cancer cells form protrusive structures identified as invadopodia by their enrichment of MT1-MMP, cortactin, Tks4, and importantly Tks5, which localizes exclusively to invadopodia. Cancer cells extend invadopodia through the endothelium into the extravascular stroma prior to their extravasation at endothelial junctions. Genetic or pharmacological inhibition of invadopodia initiation (cortactin), maturation (Tks5), or function (Tks4) resulted in an abrogation of cancer cell extravasation and metastatic colony formation in an experimental mouse lung metastasis model. This provides direct evidence of a functional role for invadopodia during cancer cell extravasation and distant metastasis and reveals an opportunity for therapeutic intervention in this clinically important process.

PubMed

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Prostate Cancer Canada’s Step Up Challenge 2019!

Prostate Cancer Canada’s Step Up Challenge 2019 pits endurance and drive against multistory office tower stairwells and gravity to raise funds for local prostate cancer research projects and initiatives in four Canadian cities. Heroic participants challenged the stairs in Toronto and Calgary on March 3rd, still to come is Vancouver on March 10th and Edmonton on March 17th (St. Patricks day!).

Congratulations to Calgary’s Step Up Challenge event participants who raised 111% of their $95 000 goal! These proceeds will support Dr. Tarek Bismar’s team at the University of Calgary. They are using blood samples from men with slow-growing prostate cancer that has not spread outside the prostate to determine if their disease is likely to advance. Learn more about this project from APCaRI and the Prostate cancer Collaborative Research Alliance.

Edmonton’s Step Up Challenge event is actively raising funds to support Dr. Kerry Courneya’s team at the University of Alberta, with 30% of the $65 000 goal reached currently. Dr. Courneya is a Professor and Canada Research Chair in Physical Activity and Cancer and Director of The Behavioral Medicine Laboratory and Fitness Center. This project is being led by doctoral student Dong-woo (Derek) Kang and will study whether exercise can reduce tumour growth and anxiety for men on active surveillance. Learn more about this project that could contribute to delaying, or even eliminating, the need for treatment from the Behavioral Medicine Laboratory and Fitness Center.

Dr. Kerry Courneya

ERASE-Scientific-Abstract

Please support the Edmonton Step Up Challenge 2019 as either a participant, or donor, or both!
The Lewis lab has entered Team Nanosteps into the challenge, check out our fundraising page and support the team by clicking on this link and making a secure online donation using your credit card.

Kerry Courneya’s Behavioral Medicine Lab team is also hitting the stairs, please click here to go to their Step Up Challenge team page to sponsor them with a donation as well!

Thanks in advance for any support you are able to provide. Get your runners and see you on March 17th!

- Perrin Beatty