Publications

Publications

Comparing efficiency of micro-RNA and mRNA biomarker liberation with microbubble-enhanced ultrasound exposure.

By:
Contributors: Robert Paproski, PhD
Ultrasound Med Biol. 2014 Sep;40(9):2207-16. doi: 10.1016/j.ultrasmedbio.2014.05.005. Epub 2014 Jul 9.

Abstract

Blood biomarkers are potentially powerful diagnostic tools that are limited clinically by low concentrations, the inability to determine biomarker origin and unknown patient baseline. Recently, ultrasound has been shown to liberate proteins and large mRNA biomarkers, overcoming many of these limitations. We have since demonstrated that adding lipid-stabilized microbubbles elevates mRNA concentration an order of magnitude compared with ultrasound without microbubbles, in vitro. Unfortunately the large size of some mRNA molecules may limit efficiency of release and hinder efficacy as an ultrasound-liberated biomarker. We hypothesize that smaller molecules will be released more efficiently with ultrasound than larger molecules. Although investigation of large libraries of biomarkers should be performed to fully validate this hypothesis, we focus on a small subset of mRNA and micro-RNAs. Specifically, we focus on miR-21 (22 base pairs [bp]), which is upregulated in certain forms of cancer, compared with previously investigated mammaglobin mRNA (502 bp). We also report release of micro-RNA miR-155 (22 bp) and housekeeping rRNA S18 (1869 bp). More than 10 million additional miR-21 copies per 100,000 cells are released with ultrasound-microbubble exposure. The low- molecular-weight miR-21 proved to be liberated 50 times more efficiently than high-molecular-weight mammaglobin mRNA, releasing orders of magnitude more miR-21 than mammaglobin mRNA under comparable conditions.

 PubMed

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APCaRI Fall Symposium Oct. 26 to 27, 2018, Banff, Alberta

2018 APCaRI Fall Symposium – Knowledge, Action, Impact

On October 26 – 27, 2018 APCaRI will celebrate its 11th research meeting at the Banff Park Lodge, in Banff, Alberta. Previous fall symposia have had over 60 participants participating in this fun and enriching event, including clinicians, scientists, clinical research personnel, trainees, benefactors and representatives of PCa support groups. Plan on attending the 2018 Fall symposium to discuss and share ideas and enjoy the beautiful Rockies!

Please submit your abstracts to Rume at djebah@ualberta.ca. Please let Rume know as soon as possible if you cannot attend. The APCaRI Fall symposium is generously supported by the Alberta Cancer Foundation and its’ donors

Dates to remember:

Trainee abstract submission deadline is September 10

Registration deadline is September 14

Submit abstracts to djebah@ualberta.ca.

APCaRI Fall symposium site- Banff Park Lodge
Banff Park Lodge

 

 

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