Assistant Professor, Applied Biostatistician
University of Alberta, Edmonton, AB
Dr. Broadhurst completed his PhD training in Biostatistics and Machine Learning at the University of Wales in Aberystwyth, UK in 1997. Dr. Broadhurst is an internationally recognized expert specializing in Experimental Design (DoE), Signal Processing, Statistics, Multivariate Data Analysis, Data Visualization, and Bioinformatics. He investigates metabolomics-the systematic, and data-driven, study of temporal interactions between the compliment of low molecular weight (bio)chemicals (metabolites) abundant within living organisms, tissues and cells. His research focuses on the development of expert systems merging clinical and ‘omic biomarker (genomics; transcriptomics; proteomics; metabolomics) measurements into clinically relevant diagnostic tools. Translating basic science into personalized (or precision) medicine.
Relevant Publications
Xia J, Broadhurst DI, Wilson M, Wishart DS: Translational biomarker discovery in clinical metabolomics: an introductory tutorial. Metabolomics 2013, 9(2):280-299.
Reinke SN, Walsh BH, Boylan GB, Sykes BD, Kenny LC, Murray DM, Broadhurst DI: (1)H NMR derived metabolomic profile of neonatal asphyxia in umbilical cord serum: implications for hypoxic ischemic encephalopathy. J Proteome Res 2013, 12(9):4230-4239.
Kirwan JA, Broadhurst DI, Davidson RL, Viant MR: Characterising and correcting batch variation in an automated direct infusion mass spectrometry (DIMS) metabolomics workflow. Anal Bioanal Chem 2013, 405(15):5147-5157.
Xia J, Mandal R, Sinelnikov IV, Broadhurst D, Wishart DS: MetaboAnalyst 2.0–a comprehensive server for metabolomic data analysis. Nucleic Acids Res 2012, 40(Web Server issue):W127-133.
Walsh BH, Broadhurst DI, Mandal R, Wishart DS, Boylan GB, Kenny LC, Murray DM: The metabolomic profile of umbilical cord blood in neonatal hypoxic ischaemic encephalopathy. PLoS One 2012, 7(12):e50520.
Reyes LM, Garcia RG, Ruiz SL, Broadhurst D, Aroca G, Davidge ST, Lopez-Jaramillo P: Angiogenic imbalance and plasma lipid alterations in women with preeclampsia from a developing country. Growth Factors 2012, 30(3):158-166.
Reinke SN, Broadhurst DI: Moving metabolomics from a data-driven science to an integrative systems science. Genome Med 2012, 4(11):85.
Egan N, Bartels A, Khashan AS, Broadhurst DI, Joyce C, O’Mullane J, O’Donoghue K: Reference standard for serum bile acids in pregnancy. Bjog 2012, 119(4):493-498.
Dunn WB, Wilson ID, Nicholls AW, Broadhurst D: The importance of experimental design and QC samples in large-scale and MS-driven untargeted metabolomic studies of humans. Bioanalysis 2012, 4(18):2249-2264.
Bartels A, Egan N, Broadhurst DI, Khashan AS, Joyce C, Stapleton M, O’Mullane J, O’Donoghue K: Maternal serum cholesterol levels are elevated from the 1st trimester of pregnancy: a cross-sectional study. J Obstet Gynaecol 2012, 32(8):747-752.
Horgan RP, Broadhurst DI, Walsh SK, Dunn WB, Brown M, Roberts CT, North RA, McCowan LM, Kell DB, Baker PN et al: Metabolic profiling uncovers a phenotypic signature of small for gestational age in early pregnancy. J Proteome Res 2011, 10(8):3660-3673.
Dunn WB, Broadhurst DI, Atherton HJ, Goodacre R, Griffin JL: Systems level studies of mammalian metabolomes: the roles of mass spectrometry and nuclear magnetic resonance spectroscopy. Chem Soc Rev 2011, 40(1):387-426.
Dunn WB, Broadhurst D, Begley P, Zelena E, Francis-McIntyre S, Anderson N, Brown M, Knowles JD, Halsall A, Haselden JN et al: Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nat Protoc 2011, 6(7):1060-1083.
Kenny LC, Broadhurst DI, Dunn W, Brown M, North RA, McCowan L, Roberts C, Cooper GJ, Kell DB, Baker PN: Robust early pregnancy prediction of later preeclampsia using metabolomic biomarkers. Hypertension 2010, 56(4):741-749.
Horgan RP, Broadhurst DI, Dunn WB, Brown M, Heazell AE, Kell DB, Baker PN, Kenny LC: Changes in the metabolic footprint of placental explant-conditioned medium cultured in different oxygen tensions from placentas of small for gestational age and normal pregnancies. Placenta 2010, 31(10):893-901.
Zelena E, Dunn WB, Broadhurst D, Francis-McIntyre S, Carroll KM, Begley P, O’Hagan S, Knowles JD, Halsall A, Wilson ID et al: Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum. Anal Chem 2009, 81(4):1357-1364.
Ong KR, Sims AH, Harvie M, Chapman M, Dunn WB, Broadhurst D, Goodacre R, Wilson M, Thomas N, Clarke RB et al: Biomarkers of dietary energy restriction in women at increased risk of breast cancer. Cancer Prev Res (Phila) 2009, 2(8):720-731.
Dunn WB, Brown M, Worton SA, Crocker IP, Broadhurst D, Horgan R, Kenny LC, Baker PN, Kell DB, Heazell AE: Changes in the metabolic footprint of placental explant-conditioned culture medium identifies metabolic disturbances related to hypoxia and pre-eclampsia. Placenta 2009, 30(11):974-980.
Brown M, Dunn WB, Dobson P, Patel Y, Winder CL, Francis-McIntyre S, Begley P, Carroll K, Broadhurst D, Tseng A et al: Mass spectrometry tools and metabolite-specific databases for molecular identification in metabolomics. Analyst 2009, 134(7):1322-1332.
Begley P, Francis-McIntyre S, Dunn WB, Broadhurst DI, Halsall A, Tseng A, Knowles J, Goodacre R, Kell DB: Development and performance of a gas chromatography-time-of-flight mass spectrometry analysis for large-scale nontargeted metabolomic studies of human serum. Anal Chem 2009, 81(16):7038-7046.
Winder CL, Dunn WB, Schuler S, Broadhurst D, Jarvis R, Stephens GM, Goodacre R: Global metabolic profiling of Escherichia coli cultures: an evaluation of methods for quenching and extraction of intracellular metabolites. Anal Chem 2008, 80(8):2939-2948.
Kenny LC, Broadhurst D, Brown M, Dunn WB, Redman CW, Kell DB, Baker PN: Detection and identification of novel metabolomic biomarkers in preeclampsia. Reprod Sci 2008, 15(6):591-597.
Dunn WB, Broadhurst D, Ellis DI, Brown M, Halsall A, O’Hagan S, Spasic I, Tseng A, Kell DB: A GC-TOF-MS study of the stability of serum and urine metabolomes during the UK Biobank sample collection and preparation protocols. Int J Epidemiol 2008, 37 Suppl 1:i23-30.
Dunn WB, Broadhurst D, Brown M, Baker PN, Redman CW, Kenny LC, Kell DB: Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. J Chromatogr B Analyt Technol Biomed Life Sci 2008, 871(2):288-298.
O’Hagan S, Dunn WB, Knowles JD, Broadhurst D, Williams R, Ashworth JJ, Cameron M, Kell DB: Closed-loop, multiobjective optimization of two-dimensional gas chromatography/mass spectrometry for serum metabolomics. Anal Chem 2007, 79(2):464-476.
Goodacre R, Broadhurst D, Smilde AK, Kristal BS, Baker JD, Beger R, Bessant C, Connor S, Capuani G, Craig A: Proposed minimum reporting standards for data analysis in metabolomics. Metabolomics 2007, 3(3):231-241.
Ellis DI, Broadhurst D, Rowland JJ, Goodacre R: Rapid detection method for microbial spoilage using FT-IR and machine learning. Rapid Methods (for Food and Feed Quality Determination) Wageningen Academic Publishers, Wageningen, the Netherlands 2007:73-84.
Elliott GN, Worgan H, Broadhurst D, Draper J, Scullion J: Soil differentiation using fingerprint Fourier transform infrared spectroscopy, chemometrics and genetic algorithm-based feature selection. Soil Biology and Biochemistry 2007, 39(11):2888-2896.
Dunn WB, Broadhurst DI, Deepak SM, Buch MH, McDowell G, Spasic I, Ellis DI, Brooks N, Kell DB, Neyses L: Serum metabolomics reveals many novel metabolic markers of heart failure, including pseudouridine and 2-oxoglutarate. Metabolomics 2007, 3(4):413-426.
Castrillo JI, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DC, Cornell MJ, Petty J, Hakes L, Wardleworth L et al: Growth control of the eukaryote cell: a systems biology study in yeast. J Biol 2007, 6(2):4.
Underwood BR, Broadhurst D, Dunn WB, Ellis DI, Michell AW, Vacher C, Mosedale DE, Kell DB, Barker RA, Grainger DJ et al: Huntington disease patients and transgenic mice have similar pro-catabolic serum metabolite profiles. Brain 2006, 129(Pt 4):877-886.
Jarvis RM, Broadhurst D, Johnson H, O’Boyle NM, Goodacre R: PYCHEM: a multivariate analysis package for python. Bioinformatics 2006, 22(20):2565-2566.
Harrison B, Ellis J, Broadhurst D, Reid K, Goodacre R, Priest FG: Differentiation of peats used in the preparation of malt for Scotch whisky production using Fourier transform infrared spectroscopy. Journal of the Institute of Brewing 2006, 112(4):333-339.
Gidman EA, Stevens CJ, Goodacre R, Broadhurst D, Emmett B, Gwynn-Jones D: Using metabolic fingerprinting of plants for evaluating nitrogen deposition impacts on the landscape level. Global Change Biology 2006, 12(8):1460-1465.
Broadhurst DI, Kell DB: Statistical strategies for avoiding false discoveries in metabolomics and related experiments. Metabolomics 2006, 2(4):171-196.
Vaidyanathan S, Jones D, Broadhurst DI, Ellis J, Jenkins T, Dunn WB, Hayes A, Burton N, Oliver SG, Kell DB: A laser desorption ionisation mass spectrometry approach for high throughput metabolomics. Metabolomics 2005, 1(3):243-250.
Ellis DI, Broadhurst D, Clarke SJ, Goodacre R: Rapid identification of closely related muscle foods by vibrational spectroscopy and machine learning. Analyst 2005, 130(12):1648-1654.
Johnson HE, Broadhurst D, Kell DB, Theodorou MK, Merry RJ, Griffith GW: High-throughput metabolic fingerprinting of legume silage fermentations via Fourier transform infrared spectroscopy and chemometrics. Appl Environ Microbiol 2004, 70(3):1583-1592.
Ellis DI, Broadhurst D, Goodacre R: Rapid and quantitative detection of the microbial spoilage of beef by Fourier transform infrared spectroscopy and machine learning. Analytica Chimica Acta 2004, 514(2):193-201.
Allen J, Davey HM, Broadhurst D, Rowland JJ, Oliver SG, Kell DB: Discrimination of modes of action of antifungal substances by use of metabolic footprinting. Appl Environ Microbiol 2004, 70(10):6157-6165.
Vaidyanathan S, Broadhurst DI, Kell DB, Goodacre R: Explanatory optimization of protein mass spectrometry via genetic search. Anal Chem 2003, 75(23):6679-6686.
Kaderbhai NN, Broadhurst DI, Ellis DI, Goodacre R, Kell DB: Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT-IR and direct injection electrospray mass spectrometry. Comp Funct Genomics 2003, 4(4):376-391.
Johnson HE, Broadhurst D, Goodacre R, Smith AR: Metabolic fingerprinting of salt-stressed tomatoes. Phytochemistry 2003, 62(6):919-928.
Allen J, Davey HM, Broadhurst D, Heald JK, Rowland JJ, Oliver SG, Kell DB: High-throughput classification of yeast mutants for functional genomics using metabolic footprinting. Nature biotechnology 2003, 21(6):692-696.
McGovern AC, Broadhurst D, Taylor J, Kaderbhai N, Winson MK, Small DA, Rowland JJ, Kell DB, Goodacre R: Monitoring of complex industrial bioprocesses for metabolite concentrations using modern spectroscopies and machine learning: application to gibberellic acid production. Biotechnol Bioeng 2002, 78(5):527-538.
Ellis DI, Broadhurst D, Kell DB, Rowland JJ, Goodacre R: Rapid and quantitative detection of the microbial spoilage of meat by fourier transform infrared spectroscopy and machine learning. Appl Environ Microbiol 2002, 68(6):2822-2828.
Raamsdonk LM, Teusink B, Broadhurst D, Zhang N, Hayes A, Walsh MC, Berden JA, Brindle KM, Kell DB, Rowland JJ et al: A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations. Nature biotechnology 2001, 19(1):45-50.
Shaw AD, Winson MK, Woodward AM, McGovern AC, Davey HM, Kaderbhai N, Broadhurst D, Gilbert RJ, Taylor J, Timmins EM et al: Rapid analysis of high-dimensional bioprocesses using multivariate spectroscopies and advanced chemometrics. Adv Biochem Eng Biotechnol 2000, 66:83-113.
Goodacre R, McGovern AC, Timmins EM, Winson MK, Kaderbhai N, Broadhurst D, Taylor J, Gilbert R, Rowland JJ, Kell DB: Intelligent systems for the characterization and quantification of microbial systems from advanced analytical techniques. In: Photonics East’99: 1999: International Society for Optics and Photonics; 1999: 174-184.
Broadhurst D, Goodacre R, Jones A, Rowland JJ, Kell DB: Genetic algorithms as a method for variable selection in multiple linear regression and partial least squares regression, with applications to pyrolysis mass spectrometry. Analytica Chimica Acta 1997, 348(1):71-86.