Assistant Professor
Alberta RNA Research and Training Institute, University of Lethbridge
Dr. Trushar R. Patel is a recent addition to the Alberta RNA Research and Training Institute and the Department of Chemistry and Biochemistry, University of Lethbridge. Dr. Patel graduated with BSc and MSc degrees in Biotechnology from the Sardar Patel University, India followed by Ph.D. studies at the University of Nottingham, United Kingdom performing biophysical characterizations of therapeutically important polysaccharides. In 2007, he joined the laboratory of Dr. Jörg Stetefeld (University of Manitoba) where he characterised extracellular matrix proteins to establish a link between their structures and functions with the support of postdoctoral fellowships from the Manitoba Institute of Child Health (2008-2010) and CIHR (2010-2012). Dr. Patel also worked in the McKenna Laboratory (2012-2013), where he studied interactions between host proteins and viral RNA molecules to understand how the terminal regions of viral RNAs recognize host proteins. In 2013, Dr. Patel was successful in receiving a distinguished Marie Skłodowska-Curie International Incoming Fellowship to understand the role of a focal adhesion proteins in cancer cell signalling using cell biology techniques at the University of Birmingham, United Kingdom.
Dr. Patel returned to Canada in 2015 to establish a Medicinal Biophysics Laboratory as part of Alberta RNA Research and Training Institute at the University of Lethbridge. His current research is focussed on studying multi-domain proteins, RNA-protein and protein-protein complexes that affect various cellular processes and diseases, especially the role of focal adhesion proteins in prostate and breast cancer. His background working with molecules from polysaccharides to nucleic acids and experience in techniques from cell biology to structure biology allows him to connect dots from genes to function. Such information can lead to the development of novel inhibitors that interfere with host-viral component interactions and therefore inhibit viral replication. Dr. Patel is also associated as a co-Director with the DiscoveryLab (the University of Alberta) that supports collaborative efforts from researchers with a wide range of background to discover and develop novel therapeutic agents. In addition, he is also serving as an Assistant Professor (adjunct) at the Cumming School of Medicine, University of Calgary.
He has published 47 peer-reviewed articles (h-index of 15) in prestigious journals with 21 as a first author (*) and 10 as a corresponding author (#) since his first article in 2006.
Relevant Publications
47. Nano-scale assembly of high-mobility group AT-hook 2a protein with DNA replication fork.
Krahn, N. Meier, M., To, V., Booy, E. P., McEleney, K., O’Neil, J. McKenna, S. A., Patel, T. R.# and Stetefeld, J.
Biophysical Journal (in press) xx, xxx-xxx PubMed
46. Impact of the structural integrity of the three-way junction of adenovirus VAI RNA on PKR inhibition.
Dzananovic, E., Astha., Chojnowski, G., Deo, S., Booy, E. P., McEleney, K., Bujnicki, J. M., Booy, E. P., Bujnicki, J. M., Patel, T. R.# and McKenna, S. A.
PLoS One (2017) 12, e0186849, 1-21 PubMed
45. Allowance for radial dilution in evaluating the concentration dependence of sedimentation coefficients for globular proteins.
Patel, T. R.#, Winzor, D. J. and Scott, D. J.
European Biophysics Journal (in press) xx, xxx-xxx PubMed
44. Intrinsic disorder in the partitioning protein KorB persists after cooperative complex formation with operator DNA and KorA.
Hyde, E. I., Callow, P., Rajesekar K. V., Timmins, P., Patel, T. R., Siligardi, G., Hussain, R., White, S. A., Thomas, C. M., and Scott, D. J.
Biochemical Journal (2017) 474, 3121–3135 PubMed
43. Structural Studies of RNA-protein Complexes: A Hybrid Approach Involving Hydrodynamics, Scattering, and Computational Methods.
Patel, T. R.#, Chojnowski, G., A., Koul, A., McKenna, S. A. and Bujnicki, J. M.
Methods (2017) 118-119, 146-162 PubMed
42. Inhibition of glycosylation on a camelid antibody uniquely affects its FcγRI binding activity.
Krahn, N., Spearman, M, Meier, M., Spearman, M., Dorion-Thibaudeau, J., McDougall, M., Patel, T. R., De Crescenzo, G., Durocher, Y., Stetefeld, J. and Butler, M.
European Journal of Pharmaceutical Sciences (2017) 96, 428-439 PubMed
2016
41. Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes.
Reuten R.*, Patel, T. R.*, McDougall, M., Rama, N., Nikodemus, D., Gibert, B., Delcros, J., Prein, C., Meier, M., Metzger, S., Zhou, Z., Kaltenberg, J., McKee, K. K., Bald, T., Tüting, T., Zigrino, P., Djonov, V., Bloch, W, Clausen-Schaumann, H., Poschl, E., Yurchenco, P. D., Ehrba, M., Mehlen, P., Stetefeld, J. and Koch, M.
Nature Communications (2016) 7, 13515, 1-17 PubMed
40. Dynamic Light Scattering: A practical guide and applications in biomedical sciences.
Stetefeld, J., McKenna, S. A. and Patel, T. R.#
Biophysical Reviews (2016) 8, 409–427 DOI
39. Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans.
Gillis, R. B., Adams, G. G., Besong, T. M., Machová, E., Ebringerova, A., Rowe, A. J., Harding S. E. and Patel, T. R.#
European Biophysics Journal (2016), 1-11 PubMed
38. LAR protein tyrosine phosphatase regulates focal adhesions via CDK1.
Sarhan, A. R., Patel, T. R., Cowell, A. R., Tomlinson, M. G., Hellberg, C., Heath, J. K., Cunningham, D. L. and Hotchin, N. A.
Journal of Cell Science (2016) 129, 2962-2971 PubMed
37. Regulation of platelet derived growth factor signaling by leukocyte common antigen-related (LAR) protein tyrosine phosphatase: A quantitative phosphoproteomics study.
Sarhan, A. R., Patel, T. R., Creese, A. J., Tomlinson, M. G., Hellberg, C., Heath, J. K., Hotchin, N. A. and Cunningham, D. L.
Molecular & Cellular Proteomics (2016) 15, 1823-1836 PubMed
36. Maltose-Binding Protein (MBP), a secretion-enhancing tag for mammalian protein expression systems.
Reuten, R., Nikodemus, D., Oliveria, M. B., Patel, T. R., Brachvogel, B., Breloy, I., Stetefeld, J. and Koch, M.
PLoS One (2016) 1(3):e0152386 PubMed
35. Structural decoding of the Netrin-1-UNC5 interaction and its therapeutical implications in cancers.
Grandina, M., Meier, M., Delcrosa, J. G., Nikodemus, D., Reuten, R., Patel, T. R., Goldschneider, D., Orriss, G., Krahn, N., Boussouar, A., Abes, R., Dean, Y., Neves, D., Bernet, A., Depil, S., Schneiders, F., Poole, K., Dante, R., Koch, M., Mehlen, P. and Stetefeld, J.
Cancer Cell (2016) 29, 175-185 PubMed
34. Biophysical analysis of a lethal laminin alpha-1 mutation reveals altered self-interaction.
Patel, T. R.#, Nikodemus, D., Reuten, R., Besong, T. M., Meier, M., Winzor, D. J., Koch, M. and Stetefeld, J.
Matrix Biology (2016) 49, 93-105 PubMed
33. Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution.
Patel, T. R.#, Winzor, D. J. and Scott, D. J.
Methods (2016) 95, 55-61 PubMed
32. Biophysical characterization of G-quadruplex recognition in the PITX1 mRNA by the specificity domain of the helicase RHAU.
Ayiro, E. O., Booy, E. P., Patel, T. R., Dzananovic, E., McRae, E. K., Meier, M., McEleney, K., Stetefeld, J. and McKenna, S. A.
PLoS ONE (2015) 10, e0144510 PubMed
31. Characterization of the termini of the West Nile virus genome and their interactions with the small isoform of the 2′ 5′-oligoadenylate synthetase family.
Deo, S., Patel, T. R., Chojnowski, G., Koul, A., Dzananovic, E., McEleney, K., Bujnicki, J. M. and McKenna, S. A.
Journal of Structural Biology (2015) 190, 236-249 PubMed
30. The β – lactamase gene regulator AmpR is a tetramer that recognizes and binds the D-Ala-D-Ala motif of its repressor UDP-N acetylmuramic acid (MurNAc)-pentapeptide.
Vadlamani, G., Balcewich, M. D., Patel, T. R., Donald, L, J., Reeve, T. M., Stetefeld, J., Standing, K. G., Vocadlo, D. J. and Mark, B. L.
Journal of Biological Chemistry (2015) 290, 2630-2643 PubMed
29. Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity.
Kumar, S., Žigman, M., Patel, T. R., Trageser, B., Gross, J. C., Rahm, K., Boutros, M., Gradl, D., Steinbeisser, H., Holstein, T., Stetefeld, J. and Özbek, S.
BMC Biology (2014) 12, 44 PubMed
28. Activation of 2’ 5’-oligoadenylate synthetase by stem loops at the 5’-end of the West Nile virus genome.
Deo, S., Patel, T. R., Dzananovic, E., Booy, E. P., Zeid, K., McEleney, K., Harding, S. E. and McKenna, S. A.
PLoS ONE (2014) 9, e92545 PubMed
27. Conformational itinerary of Pseudomonas aeruginosa 1,6-Anhydro-N-acetylmuramic acid kinase during its catalytic cycle.
Bacik J. P., Tavassoli. M., Patel, T. R., McKenna, S. A., Vocadlo, D. J., Khajehpour, M. and Mark, B. L.
Journal of Biological Chemistry (2014) 289, 3176-3185 PubMed
26. Solution conformation of adenovirus virus associated RNA-I and its interaction with PKR.
Dzananovic, E.,* Patel, T. R.*, Chojnowski, G., Boniecki, M. J., Deo, S., McEleney, K., Harding, S. E., Bujnicki, J. M. and McKenna, S. A.
Journal of Structural Biology (2014) 185, 48-57 PubMed
25. Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution.
Patel, T. R., Bernards, C., Meier, M., McEleney, K., Winzor, D. J., Koch, M. and Stetefeld, J.
Matrix Biology (2014) 33, 60-67 PubMed
24. Binding of G-quadruplexes to the N-terminal recognition domain of the RNA helicase associated with AU-rich element (RHAU).
Meier, M.,* Patel, T. R.*, Booy, E. P., Marushchak, O., Okun, N., Deo, S., Howard, R., McEleney, K., Harding, S. E., Stetefeld, J. and McKenna, S. A.
Journal of Biological Chemistry (2013) 288, 35014-35027 PubMed
23. C1q-tumour necrosis factor-related protein 8 (CTRP8) is a novel interaction partner of relaxin receptor RXFP1 in human brain cancer cells.
Glogowska, A., Kunanuvat, U., Stetefeld. J., Patel, T. R., Thanasupawat, T., Krcek, J., Weber, E., Wong, G. W., Bigio, M., Hoang-Hu, C., Hombach-Klonisch, S., Klonisch, T.
The Journal of Pathology (2013) 231, 466-479 PubMed
22. A potential for overestimating the absolute magnitudes of second virial coefficients by small-angle X-ray scattering.
Scott, D. J., Patel, T. R. and Winzor, D. J.
Analytical Biochemistry (2013) 435, 159-165 PubMed
21. Recognition of viral RNA stem-loops by the tandem double-stranded RNA binding domains of PKR.
Dzananovic, E.,* Patel, T. R.*, Deo, S., McEleney, K., Stetefeld, J. and McKenna, S. A.
RNA (2013) 19, 333-344 PubMed
20. Site specific cleavage mediated by MMPs regulates function of agrin.
Patel, T. R., Butler, G., McFarlane, A., Xie, I., Overall, C. M. and Stetefeld, J..
PLoS ONE (2012) 7, e43669 PubMed
19. Determination of a molecular shape for netrin-4 from hydrodynamic and small angle X-ray scattering measurements.
Patel, T. R., Reuten, R., Xiong, S., Meier, M., Winzor, D. J., Koch, M. and Stetefeld, J.
Matrix Biology (2012) 31, 135-140 PubMed
18. The macromolecular state of A-kinase anchoring protein.
Patel, T. R.# and Winzor, D. J.
Journal of Molecular Recognition (2012) 25, 11-14 PubMed
17. Evidence for self-association of a miniaturized version of agrin from hydrodynamic and small-angle X-ray scattering measurements.
Patel, T. R.#, Besong, T. M., Patel, N., Meier, M., Harding, S. E., Winzor, D. J. and Stetefeld, J.
Journal of Physical Chemistry B (2011) 115, 11286-11293 PubMed
16. Examination of the discrepancy between size estimates for ovalbumin from small-angle X-ray scattering and other physicochemical measurements.
Scott, D. J., Patel, T. R., Besong, T. M., Stetefeld, J. and Winzor, D. J.
Journal of Physical Chemistry B (2011) 115, 10725-10729 PubMed
15. T-shaped arrangement of the recombinant agrin G3-IgG Fc protein.
Patel, T. R., Meier, M., Li, J. Morris, G., Rowe, A. J. and Stetefeld, J.
Protein Science (2011) 20, 931-940 PubMed
14. Reassessment of the size of the supermolecular state of Dishevelled-3.
Patel, T. R.# and Winzor, D. J.
Journal of Molecular Recognition (2011) 24, 843-846 PubMed
13. Nano-structure of the laminin γ-1 short arm reveals an extended and curved multidomain assembly.
Patel, T. R., Morris, G. A., Zwolanek, D., Keene, D. R., Li, J., Harding, S. E., Koch, M. and Stetefeld, J.
Matrix Biology (2010) 29, 565-572 PubMed
12. Absence of a catalytic water confers resistance to the neurotoxin gabaculine.
Orriss, G. L., Patel, T. R., Sorensen, J. and Stetefeld, J.
FASEB Journal (2010) 24, 404-414 PubMed
11.Vimentin coil 1A-A molecular switch involved in the initiation of filament elongation.
Meier, M., Padilla, G. P., Herrmann, H., Wedig, T., Hergt, M., Patel, T. R., Stetefeld, J., Aebi, U. and Burkhard, P.
Journal of Molecular Biology (2009) 390, 245-261 PubMed
10. Molecular flexibility of methylcelluloses of differing degree of substitution by combined sedimentation and viscosity analysis.
Patel, T. R., Morris, G. A., Garcia de la Torre, J., Ortega, A., Mischnick, P. and Harding, S. E.
Macromolecular Bioscience (2008) 8, 1108-1115 PubMed
09. Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells.
Inngjerdingen, M., Inngjerdingen, K. T., Patel, T. R., Allen, S., Chen, X., Rolsted, B., Harding, S. E., Michaelsen, T. J., Diallo, D. and Paulsen, B. S.
Glycobiology (2008) 18, 1074-1084 PubMed
08. Global conformation analysis of irradiated xyloglucans.
Patel, T. R., Ebringerová, A., Vodeničarová, M., Velebny, V., Morris, G. A., Ortega, A., Garcia de la Torre, J. and Harding, S. E.
Carbohydrate Polymers (2008) 74, 845-851 DOI
07. Global hydrodynamic analysis of the molecular flexibility of galactomannans.
Morris, G. A., Patel, T. R., Picout, D. R., Ross-Murphy, S. B., Ortega, A., Garcia de la Torre, J. and Harding, S. E.
Carbohydrate Polymers (2008) 72, 356-360 DOI
06. Immunological and structural properties of a pectic polymer from Glinus oppositifolius.
Inngjerdingen, K. T., Patel, T. R., Chen, X., Lennart, K., Allen, S., Morris, G. A., Harding, S. E., Matsumoto, T., Diallo, D., Yamada, H., Michaelsen, T. E., Inngjerdingen, M. and Paulsen, B. S.
Glycobiology (2007) 17, 1299-1310 PubMed
05. Weak self-association in a carbohydrate system.
Patel, T. R., Harding, S. E., Ebringerová, A., Deszczynski, M., Hromadkova, Z., Togola, A., Paulsen, B. S., Morris, G. A. and Rowe, A. J.
Biophysical Journal (2007) 93, 741-749 PubMed
04. Pressure cell assisted solution characterization of galactomannans. 3. Application of analytical ultracentrifugation techniques.
Patel, T. R., Picout, D. R., Harding, S. E. and Ross-Murphy, S. B.
Biomacromolecules (2006) 7, 3513- 3520 PubMed
03. Resonance energy transfer between green fluorescent protein variants: complexities revealed with myosin fusion proteins.
Zeng, W., Seward, H. E., Málnási-Csizmadia, A., Wakelin, S., Woolley, R. J., Cheema, G. S., Basran, J., Patel, T. R., Rowe, A. J. and Bagshaw, C. R.
Biochemistry (2006) 45, 10482-10491 PubMed
02. Damaged starch characterisation by ultracentrifugation.
Tester, R. F., Patel, T. R. and Harding, S. E.
Carbohydrate Research (2006) 341, 130-137 PubMed
01. Structures and structure-activity relationships of three mitogenic and complement fixing pectic arabinogalactans from the malian antiulcer plants Cochlospermum tinctorium A. Rich and Vernonia kotschyana Sch. Bip. ex Walp.
Nergard, C. S., Kiyohara, H., Reynolds, J. C., Thomas-Oates, J. E., Matsumoto, T., Yamada, H., Patel, T. R., Petersen. D., Michaelsen, T. E., Diallo, D., Paulsen, S. B.
Biomacromolecules (2006) 7, 71-79 PubMed